C9017

Sigma

 

Concanavalin A from Canavalia ensiformis (Jack bean)

Sepharose® conjugate, buffered aqueous suspension

Synonym:Con A
CAS Number:11028-71-0
MDL number:MFCD00071069

Description

Analysis NoteWhere reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
ApplicationUsed for the purification of glycoproteins containing mannose.
Biochem/physiol ActionsCon A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Physical formSuspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl2 , MgCl2, and MnCl2
Legal InformationSepharose is a registered trademark of GE Healthcare

Properties

formbuffered aqueous suspension
contains20% ethanol as preservative
extent of labeling10-15 mg per mL
matrix Sepharose 4B
matrix activationcyanogen bromide
matrix attachmentamino
matrix spacer1 atom
capacity ≥25 mg/mL binding capacity (thyroglobulin)
conjugateSepharose® conjugate
storage temp.2-8°C

Safety

Risk Statements10
RIDADRUN 1170 3/PG 3
WGK Germany2
Flash Point(F)103 °F
Flash Point(C)39 °C

Related Products

Related productC7555, Concanavalin A from Canavalia ensiformis (Jack bean)

References

referenceReeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)
 Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)
 Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)
 , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
MerckMerck 13,2521